ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the life quality of men after surgical sterilization.</p><p><b>METHODS</b>We investigated the incidence of psychosomatic reactions, their influential factors and the quality of life among 740 men after surgical sterilization. We also evaluated the effectiveness of mental intervention for those with psychosomatic reactions.</p><p><b>RESULTS</b>Totally, 59 (8%) of the subjects were not satisfied with their life status, including 42 (5.67%) suffering from psychosomatic reactions, and their psychosomatic reactions were related to their education background, economic status and understanding of sterilization. After 2 - 24 months of mental intervention, 34 (80.95%) of the 42 cases were cured, 6 (14.29%) relieved, and 2 (4.76%) unimproved.</p><p><b>CONCLUSION</b>Mental intervention is effective for psychosomatic reactions in men after surgical sterilization.</p>
Subject(s)
Adult , Humans , Male , Middle Aged , Postoperative Period , Quality of Life , Vasectomy , PsychologyABSTRACT
<p><b>AIM</b>To identify specifically expressed genes in the adult and fetal testes.</p><p><b>METHODS</b>A human testis cDNA microarray was established. Then the mRNA of adult and fetal testis was purified and probes were prepared by a reverse transcription reaction with the testis mRNA as template. The microarray was hybridized with probes of adult and fetal testes. The nucleic sequences of differentially expressed genes were determined and homologies were searched in the databases of the GenBank.</p><p><b>RESULTS</b>When hybridized with adult or fetal testis probes, the positive clones were 96.8 % and 95.4 %, respectively. Among these genes, one was a new testis-specific gene, which was named TSP1. TSP1 was highly expressed in human adult testis. The cDNA of TSP1 was 1,484 bp in length. The cDNA sequence of this clone was deposited in the Genbank (AF333098). TSP1 was also determined as Interim Gen Symbol (Unigene, No. Hs.98266). Protein analysis showed that TSP1 contained two functional domains: an N-terminal basic helix-loop-helix (bHLH) and a C-terminal leucine zipper (Zip). Homologous analysis showed that the 430 amino acid sequences deduced from the 1293 bp open reading frame (ORF) had a homology with the human gene FLJ2509 (AK098575). TSP1 had also a sequence homology with Spz 1 protein of mouse. Expression profiles showed that TSP1 was specifically and strongly expressed in the testis.</p><p><b>CONCLUSION</b>TSP1 is a gene highly expressed in adult testis. It may play an important role in spermatogenesis in the humans.</p>